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OBJECTIVE To prepare spider fibroin membrane loaded with Periplaneta americana extract， and investigate its characterization， in vitro drug release property and cytotoxicity. METHODS Using natural spider silk collected from Chilobrachys guangxiensis as raw material， P. americana extract as model drug， the drug-loaded spider fibroin membrane （hereinafter referred to as drug-loaded membrane） was prepared by solvent casting method. The material matrix spider fibroin membrane without P. americana extract （hereinafter referred to as blank membrane） was prepared with same method. The membrane structure was characterized by static water contact angle， Fourier infrared chromatography， X-ray diffraction and scanning electron microscopy from different angles； drug release characteristics in artificial saliva were simulated in vitro to evaluate the drug sustained-release performance. MTT assay was adopted to validate the cytotoxicity of drug-loaded membrane. RESULTS The drug-loaded membrane was prepared， and the static water contact angle was less than 90°， which was less than that of blank membrane. The drug-loaded membrane showed the characteristic absorption peak to polypeptide of P. americana extract at 1 500-1 700 cm－1. X-ray diffraction and scanning electron microscopy also proved that the drug was successfully loaded into the pellicle. The release time of the pellicle in artificial saliva was more than 200 min. The MTT test results showed that the cell proliferation rates of blank membrane and drug-loaded membrane were 84.6% and 79.4% （both greater than 70%）， respectively， without significant potential cytotoxicity. CONCLUSIONS Drug-loaded membrane prepared with natural spider silk has a certain sustained-release effect in artificial saliva， which can be further developed as a drug sustained-release carrier with excellent biological characteristics and biocompatibility.
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As the reserve force of traditional Chinese medicine (TCM), students in TCM colleges or universities play an important role in promoting the development of TCM. However, these students have disadvantages in humanistic quality, such as the lack of humanistic knowledge, awareness of the fear of life, and the inadequacy of professional morality, which may affect their future job performance. In view of the present situation of the humanistic quality of students in TCM colleges or universities, we analyzed the factors affecting their humanistic quality and proposed to build a platform to exchange TCM culture, enrich teachers' knowledge of TCM culture and cultivate the professional ethics of students in TCM colleges or universities, in combination with the core values of TCM culture such as profoundness, reality, benevolence, effectiveness, so as to improve students' overall humanistic quality.
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As the reserve force of traditional Chinese medicine(TCM),students in TCM colleges or universities play an important role in promoting the development of TCM.However,these students have disadvantages in humanistic quality,such as the lack of humanistic knowledge,awareness of the fear of life,and the inadequacy of professional morality,which may affect their future job performance.In view of the present situation of the humanistic quality of students in TCM colleges or universities,we analyzed the factors affecting their humanistic quality and proposed to build a platform to exchange TCM culture,enrich teachers' knowledge of TCM culture and cultivate the professional ethics of students in TCM colleges or universities,in combination with the core values of TCM culture such as profoundness,reality,benevolence,effectiveness,so as to improve students' overall humanistic quality.
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As the The Law of Traditional Chinese Medicine in People's Republic of China has been carried out, the gradual implementation and the development of traditional Chinese medicine apprentice education were increasing. This paper described the current situation and development of traditional Chinese medicine apprentice education.Based on the The Law of Traditional Chinese Medicine in People's Republic of China,the development and trendency of traditional Chinese medicine apprentice education in our country were explored, and the supporting foundation of policy of traditional Chinese medicine apprentice education were analyzed, in order to give some relevant suggestion.
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To a certain extent, precision medicine based on individualized medical treatment process, like syndrome differentiation of traditional Chinese medicine. This paper mainly analyzed the similarities and differences of theory, practice and development between precision medicine and TCM syndrome differentiation. After the analysis, we found that these two medicine body could be integrated to improve the progress of medicine albeit some differences.
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The strategy of One Belt and One Road established new era for China to open-door to the world. It is an important way to establish and strengthen the relationship among countries along the belt and the road. This paper has analyzed and shown the history, prespects and development of Chinese medicine trade between China and Russia in context of “One Belt and One Road”. And then counter measures were proposed, such as promoting diversification of Chinese medicine service, developing international standard in Chinese medicine, strengthening cultural exchanges of Chinese medicine, advertising Chinese medicine in Russia, and so on.
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The ISO9000 family standards developed by the international organization are the international quality standards which are widely used in various types and sizes of organizations.At present,the graduate student management method introduced by our country plays a certain positive role in guaranteeing the graduate students quality,but it still lacks effective quality control and has not formed the perfect quality management system.Through the analysis of the status quo of the development of graduate education and the problems need to pay attention to in the ISO9000 family standard procedure,document and other factors as well as the construction of the ISO9000 family standard,we put forward goal elaboration,regular staff training and other measures.
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This article analyzed the difference between Chinese and Russian traditional medical treatments from the part of herbs,acupuncture/acupressure therapy,manual therapy,psychotherapy,and sports.TCM had a separate comprehensive diagnosis and treatment system,Russia traditional medicine,attached to Western medicine,had not formed the independent diagnostic and treatment system.
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System combing the internal unity of traditional Chinese medicine culture and socialist core values is the demands of both contemporary TCM culture intrinsic appeal and also the practice of socialist core values. Based on exploring the inner link between the core value of traditional Chinese medicine culture and socialist core values, this article discuss the specific path of promoting socialist core values in the process of TCM culture inheritance and innovation.
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Objective To establish a method to compare the difference of exposure component of oral taking different part ofPueraria Lobata (Willd.) Ohwi in intestine.Methods After the incubation of puerarin,extraction Radix Puerariae and Flos Puerariae with S9,the mixtures were centrifuged to get the supernatant for analysis with HPLC.Results The research showed that the metabolic rate of puerarin was higher than that of extractions because of the concentration of enzyme.The difference between the fingerprints of Radix Puerariae and Flos Puerariae Lobata indicated that there was a difference of chemical components in such two parts of Kudzuvine Root.Conclusion The profile of intestinal metabolism can be revealed in the research of gut wall metabolism in the course of intestinal absorption by S9 incubation.
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Outstanding traditional culture of traditional Chinese medicine contains rich hu-manistic knowledge and is the precious spiritual resource and important education material for moral education. Heilongjiang University of Chinese Medicine gave full play of outstanding traditional cul-ture of traditional Chinese medicine in moral education and integrated it into moral education thus to innovate teaching methods and expand development room of moral education.
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Objective To evaluate the effect of vacuum sealing drainage(VSD)ON the destroyed hand injuries.Methods From January 2006 to December 2008,forty pafients with destroyed hand injuries (surface of wound)were randomly divided into VSD group(20 cases)and control group(chlorhexidine wet compress)(20 cases)after debridement. The outcomes were retrospectively analysed by pain score,swelling score,bacterial positive rate and necrotic tissue.Results Three days after treatment,the pain score of VSD group was(2.62±1.54)degree,and that of control group was(3.39±1.64)degree.There wag significant difference between the two groups(P＜0.01).The swelling score of VSD group was(1.51±0.25)degree which was different from control group(2.29±1.26)degree(P＜0.05).The bacterial positive rate of control group was 20%(4/20),but that of VSD group was 5%(1/20).There was significant difference between the two groups(P＜0.05).The VSD group had no necrotic tissue occurrence,but the control group had 2 cases,one of which had serious necrosis and had escapology.Conclusion VSD Can inhibit bacterial infection,ease pain,exempt changing dressings and stimulate growing of granulation tissue also can maintain a good environment for tissue transplantation later.So VSD combining with tissue transplantation is an ideal therapy in destroyed hand injuries.